(1996) to amplify the bacterial V6‐V8 16S rRNA gene segment. Approximately 30 adults from each of the sampled populations were dissected aseptically in a plate containing 1 ml of sterile distilled water using sterilized tweezers under a stereomicroscope. Typhlodromus bagdasarjani We chose the three bacterial suspension concentrations of 10−4, 10−5, and 10−6 as our samples and plated these onto LB agar and incubated at 37 °C for 24–48 h. All of the treatments were performed in triplicate to ensure that the growth of microorganisms was observed in a timely and accurate manner. Lobesia botrana A B. cucurbitae genetic sexing strain (GSS) was established for SIT. According to our analysis, we suggest that the jackknife technique should not be used for the estimation of variability of the net reproductive rate. Induction of Resistance Against Plutella xylostella (L.) (Lep. The oesophageal diverticulum of Dirioxa pornia studied through micro-CT scan, dissection and SEM studies. Transgenerational hormetic effects of sublethal dose of flupyradifurone on the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Impact of constant versus fluctuating temperatures on the development and life history parameters of Tetranychus urticae (Acari: Tetranychidae). Box 17‐25, Taichung, Taiwan, Republic of China. A 200‐μl aliquot from these samples was further serially diluted to 10−6. In Hawaii, establishment of Bactrocera cucurbitae (Coquillett) in 1895 resulted in the introduction of the most successful parasitoid, Psyttalia fletcheri (Silvestri); similarly, establishment of Bactrocera dorsalis (Hendel) in 1945 resulted in the introduction of 32 natural enemies of which Fopius arisanus (Sonan), Diachasmimorpha longicaudata (Ashmead) and Fopius vandenboschi (Fullaway) … The PCR reaction (25 μl) contained 0.5 μl 2× Trans Taq HiFi PCR SuperMix, 2.0 μl 10 mM dNTPs, 0.5 μl 10 mM each primer, 2.5 μl 10× PCR buffer I, 1 μl template DNA, and 18 μl ddH2O. There was no significant variation in flight ability among the different treatments (F2,6 = 0.894, P = 0.46). Acetamiprid resistance and fitness costs of melon aphid, Aphis gossypii: An age-stage, two-sex life table study. Effect of Arsenophonus Endosymbiont Elimination on Fitness of the Date Palm Hopper, Ommatissus lybicus (Hemiptera: Tropiduchidae). in Hawaii (Clausen, 1978) may have been expected to have some impact on B. cucurbitae as well as Bactrocera dorsalis. Enterobacter spec., Providencia spec., Klebsiella spec., Cedecea spec., Lysinibacillus spec., and various other bacteria were isolated. ... (Bactrocera cucurbitae) controlling Bt strains is also an important objective of this work. . Life‐history and demographic traits of the marula fruit fly Ceratitis cosyra: potential consequences of host specialization. Control Tools. Potential diet regimens for laboratory rearing of the harlequin ladybird. These results might explain how the irradiation used in SIT damages the balance of bacterial communities harbored in the target fruit fly gut (Lauzon & Potter, 2012) and illustrate that Enterobacter spec. Biological Control. This finding suggests that the nutritional benefit of bacteria had a more significant influence than the insect–bacteria interaction, consistent with B. cucurbitae females placed in an environment with no food and water (Table 1). Temperature-dependent development and life history of Oligonychus litchii (Acari: Tetranychidae), on wax apple. It has anterior mouth hooks, ventral fusiform areas and a flattened caudal end. Biological control programmes can result in significant reduction in pest populations, and such reductions have led to relatively pest-free cultivation of at least some less susceptible varieties of fruits and vegetables. Demography and Uncertainty of Population Growth of Conogethes punctiferalis (Lepidoptera: Crambidae) Reared on Five Host Plants With Discussion on Some Life History Statistics. The Incidence and the Implications of Natural Infection with However, survival rates varied significantly for the different treatments (F2,6 = 12.732, P<0.01); a greater increase was observed for treatment with API than for treatment with PI (18 vs. 12% increase). For example, the irradiation used for SIT disturbs microbe balance in the midgut for C. capitata and A. ludens, reducing the abundance of specific, functional gut bacteria (Niyazi et al., 2004; Ami et al., 2010; Estes et al., 2011; Gavriel et al., 2011; Jurkevitch, 2011; Zindel et al., 2011; Lauzon & Potter, 2012). Neither the PI treatment nor the API treatment significantly affected AL or WL in the irradiated B. cucurbitae GSS (Table 1) (female AL: F2,24 = 1.681, P = 0.26; female WL: F2,24 = 0.721, P = 0.52; male AL: F2,24 = 2.228, P = 0.19; male WL: F2,24 = 2.124, P = 0.20). The results indicate that pupal weight, survival rate, and some morphological indices were significantly increased. 2 Adults were fed a mixture of sucrose and yeast powder at a ratio of 3:1. Survival and Development of Spinosad is a natural insecticide with desirable qualities, and it is widely used as an alternative to organophosphates for control of pests such as the melon fly, Bactrocera cucurbitae (Coquillett). The full text of this article hosted at iucr.org is unavailable due to technical difficulties. The original 3120 bp hopperBd-Kah element isolated from the Kahuku wild-type strain was highly degenerate and appeared to have a mutated transposase and terminal sequences, while a second … The %emergence rate (E%) and fly‐out rate (F%) were measured: E% = 100% × [(100 − A‐B)/100], and F% = 100% × [(100 − A‐B‐C‐D)/100]. Journal of the Kansas Entomological Society. The Lesser Wax Moth 0 = No mandates. Males of the laboratory‐reared B. cucurbitae GSS emerge from brown pupae, whereas females emerge from white pupae. and an un-named Spalangia sp. . Effects of constant and stage-specific-alternating temperature on the survival, development and reproduction of the oriental armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae). Autographa gamma In the future, we can focus on discovering more suitable probiotics to strengthen fruit fly fitness and improve SIT. means to control the insects in all crops including vegetables. The dead and live flies were counted after 48 h. Nine replicates were performed per group. Fitness costs in clothianidin-resistant population of the melon aphid, Aphis gossypii. Poppius (Hemiptera: Anthocoridae) on (Farlow) Samson on the Life-Table Parameters of Laboratory-Reared ENT5, a Component of the Symbiotic Community of Tephritid Flies Sterile insect technique (SIT) is a ‘hot’ research field for pest control, particularly in fruit flies. Lethal and Sublethal Effects of Cantharidin on Development and Reproduction of Plutella xylostella (Lepidoptera: Plutellidae). Nomuraea rileyi With respect to male survival rates, the PI and API treatments produced significantly elevated survival compared with the NPI treatment (F2,6 = 13.616, P<0.01); this finding is consistent with the above results of probiotic diets on B. cucurbitae females (Table 1). (Coleoptera: Chrysomelidae), Fed on Alligator Weed Under Elevated CO International Journal of Tropical Insect Science. Loading. Six hundred micrograms of bacterial suspension in autoclave‐sterilized LB liquid medium was autoclaved at 121 °C for 20 min as a live probiotic in a 150‐ml shaker flask. Diet quality affects bait performance in German cockroaches (Dictyoptera: Blattellidae). Demographic Analysis of Sex Ratio on Population Growth of Screening of Potential Sources of Resistance to The PCR conditions were as follows: initial denaturing step of 94 °C for 5 min; followed by 34 cycles of denaturation at 94 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 1 min; and a final extension step at 72 °C for 5 min. . This means that the success of SIT may be impaired by inappropriate habitat colonization, mass‐rearing conditions, and irradiation dose (Behar et al., 2008c; Ami et al., 2010). After dissection, guts were collected in 1.5‐ml Eppendorf tubes in 1 ml of sterile phosphate‐buffered saline and homogenized. In the Solomon Islands it was subjected to an eradication campaign using a combination of bait spraying and male annihilation traps. Habrobracon hebetor International Journal of Pest Management. However, for both sexes, PI individuals were significantly larger than API and NPI individuals (Table 1) with respect to HW (female HW: F2,24 = 25.04, P<0.01; male HW: F2,24 = 5.794, P = 0.04) and TL (female TL: F2,24 = 10.17, P = 0.012; male TL: F2,24 = 16.84, P<0.01). Fitness costs associated with chlorantraniliprole resistance in (Lepidoptera: Noctuidae). Archives of Phytopathology and Plant Protection. The 16S rRNA gene sequence from each isolate was compared with 16S rRNA sequences in the GenBank database using the BLAST search algorithm (Altschul et al., 1997). Irradiation effect on the structure of bacterial communities associated with the oriental fruit fly, Bactrocera dorsalis. Myzus persicae . . Culex pipiens The mean female and male pupal weight increased by 0.17 ± 0.04 and 0.08 ± 0.01 mg, respectively (Table 1), although the autoclaved probiotic (API) did not affect pupal weight as robustly as PI. Number of times cited according to CrossRef: Bacterial symbiosis in Bactrocera oleae, an Achilles’ heel for its pest control. Entomol. Limited investigations on parasitoids of the melon fly, Bactrocera cucurbitae (Coquillett), infesting five species of Cucurbitaceae and seven species of Solanaceae were conducted in Thailand to determine natural occurrence of biological control agents. Females and males were obtained from pupae and irradiated with 100 Gy of gamma radiation from 137Caesium 2 days before emergence (Wu et al., 2015). Annals of the Entomological Society of America. Learn about our remote access options, Laboratory of Theoretical and Applied Ecology, Department of Entomology, National Chung Hsing University, Taichung, Taiwan, Republic of China, Hsin Chi (corresponding author), P.O. Bensulfuron-Methyl Treatment of Soil Affects the Infestation of Whitefly, Aphid, and Tobacco Mosaic Virus on Nicotiana tabacum. (Diptera: Tephritidae) With Discussion of Control Efficacy Using Male Annihilation Spodoptera litura These parasites lay their eggs in the eggs or maggots and emerge in the pupal stage. Name Authority; Bactrocera cucurbitae (Coquillett) Chaetodacus cucurbitae (Coquillett) Dacus aureus: Tseng & Chu: Dacus cucurbitae: Coquillett: Dacus yuiliensis accounted for 18.5, 37.0, 1.9, 24, and 1.9% of all examined sequences, respectively. The entire gut was collected from 5‐day‐old males and females from non‐irradiated B. cucurbitae GSS. Sublethal Effects of Cyantraniliprole and Imidacloprid on Feeding Behavior and Life Table Parameters of Tetranychus urticae Our results revealed significant variability between the experiments under both laboratory and field conditions; this variability should be taken into consideration in the data collection and application of life tables. A B. cucurbitae genetic sexing strain (GSS) was established for SIT. Assessing Compatibility of the Predatory Mite Heat treatment is one of the numerous effective quarantine treatments for Bactrocera flies (Heather and Hallman 2008). Agasicles hygrophila The oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is one of the major insect pests which renders the fruit to become unfit for human consumption. Chrysodeixis chalcites Bactrocera cucurbitae Coquillett, 1849 (mosca del melone) è una mosca della famiglia Tephritidae. This established strain was generated by imitating an established method of SIT for B. dorsalis (Wu et al., 2015) at the Institute of Beneficial Insects, Fujian Agricultural and Forestry University, Fuzhou, China. Sub-lethal concentrations of the entomopathogenic fungus, Beauveria bassiana increase fitness costs of Helicoverpa armigera (Lepidoptera: Noctuidae) offspring. High‐quality PCR products were sent for sequencing at Biosune Biotechnology (Shanghai, China) with an ABI 3730 XL using the 968GC and L1401 primers. To determine the effect of the probiotic‐enriched diet on pupal weight, emergence (%emergence rate) and flight ability (fly‐out rate) in irradiated B. cucurbitae females and males, pupa of each type were placed in insect‐rearing cages (1.2 × 0.6 × 1.2 m). Please check your email for instructions on resetting your password. E‐mail: hsinchi@dragon.nchu.edu.tw. Improving pest control: mass rearing and field performance – an introduction. Effects of prolonged mass rearing on life history traits of Microbiota and potential opportunistic pathogens associated with male and female fruit flies of Malaysian Bactrocera carambolae (Insecta: Tephritidae). The guidelines state that the eggs and larvae of Bactrocera must be killed in accordance with the export protocols between importing and exporting countries (MAFF-PPS 2015, USDA-APHIS-PPQ-CPHST 2015). Toxicity and sublethal effects of two plant allelochemicals on the demographical traits of cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae). These studies demonstrate the applied value of Enterobacter spec. The effects of the probiotics on pupal weight, emergence, flight ability, adult survival, HW, TL, AL, and WL were assessed with one‐way ANOVA. The nutrient effect of Enterobacter spec. Equine hepatocytes: isolation, cryopreservation, and applications to in vitro drug metabolism studies. was more significant than the insect–bacteria interaction in B. cucurbitae GSS (Behar et al., 2005), which is in accordance with our goal. Microbiome Innovation in Agriculture: Development of Microbial Based Tools for Insect Pest Management. . Hermetia illucens This result is different from that of Augustinos et al. Learn about our remote access options, Institute of Beneficial Insects, College of Plant Protection, Fujian Agriculture and Forestry University, Agricultural Entomology and Pest Control, Institute of Beneficial Insects, Fuzhou, 350002 China. The melon fruit fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae), is polyphagous and an important fruit and vegetable pest. The population growth, development and metabolic enzymes of the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae) under the sublethal dose of triflumezopyrim. Neoseiulus barkeri (Lepidoptera: Noctuidae), on six commercial tomato cultivars under laboratory conditions Reports evaluating B. cucurbitae in the context of SIT have largely focused on examining ecology, mating behavior, spatial distribution, and genetics rather than application of a probiotic to enhance performance (Ito et al., 2003; Koyama et al., 2004). PCR amplification was performed from the bacterial solution using the universal primers 968GC and L1401 described by Nubel et al. Differential abundance and core members of the bacterial community associated with wild male Zeugodacus cucurbitae fruit flies (Insecta: Tephritidae) from three geographical regions of Southeast Asia. Of these, 20 colonies were identified as Enterobacter spec., 10 as Providencia spec., 13 as Cedecea spec., 1 Lysinibacillus spec., and 1 as Klebsiella spec. The Enterobacter spec. on cucumber plants An analysis of the isolated bacteria revealed that Enterobacter spec. was utilized in luring experiments, in which baits were most attractive to Bactrocera zonata (Saunders) (Reddy et al., 2014). (Hemiptera: Aphididae) Many of these are either known or believed to have the potential to damage a diverse array of important crops. Temperature-Mediated Effects of Host Alternation on the Adaptation of Myzus persicae (Hemiptera: Aphididae). Sublethal effects of the novel cis-nitromethylene neonicotinoid cycloxaprid on the cotton aphid Aphis gossypii Glover (Hemiptera: Aphididae). Canola quality affects second (Brevicoryne brassicae) and third (Diaeretiella rapae) trophic levels. and you may need to create a new Wiley Online Library account. It was reared under controlled temperature, humidity, and light conditions (25 ± 1 °C, 65 ± 5% r.h., L12:D12 photoperiod) for >13 generations. Mass rearing and superior‐fit target species are crucial components of SIT. Fitness costs of sulfoxaflor resistance in the cotton aphid, Aphis gossypii Glover. The responses of cucumber plants subjected to different salinity or fertilizer concentrations and reproductive success of Tetranychus urticae mites on these plants. Control: Bactrocera Cucurbitae Host Crop: All Vegetables crops. ^ ARS | Publication request: Parasitization of Melon Fly (Diptera:tephritidae) by Fopius Arisanus and Psyttalia Fletcheri (Hymenoptera:braconidae) and the Effect of Fruit Substrates on Host Preference by Parasitoids , Ars.usda.gov. . Means within a row followed by the same letter do not differ significantly (one‐way ANOVA followed by Fisher's LSD test: P>0.05). Enterobacter spec. Please check your email for instructions on resetting your password. (Hymenoptera: Braconidae) Working off-campus? The effect of diet and radiation on the bacterial symbiome of the melon fly, Zeugodacus cucurbitae (Coquillett). as a PI or an API treatment did not affect the emergence of irradiated B. cucurbitae females (F2,6 = 0.939, P = 0.44). (Diptera: Tephritidae) based on Demographic Analysis of Group-Reared Life Table abundance in irradiated hosts. Demographic Parameters of When the effect of Enterobacter spec. Under Simulated Weather Conditions In Egypt Six colonies were similar to BM35 (uncultured bacteria) that had previously been isolated from the guts of adult B. tau females and males (Luo et al., 2016). Chronic Sublethal Effects of Cantharidin on the Diamondback Moth Plutella xylostella (Lepidoptera: Plutellidae). Sterile insect technique (SIT) is an effective and valuable tool for controlling infestations of various fruit flies (Diptera: Tephritidae), including the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), the Mexican fruit fly, Anastrepha ludens (Loew), and the olive fly, Bactrocera oleae (Rossi) (Ami et al., 2010; Estes et al., 2012; Lauzon & Potter, 2012; Hamden et al., 2013; Sacchetti et al., 2013; Ben‐Yosef et al., 2014; Augustinos et al., 2015). Soybean aphid (Hemiptera: Aphididae) response to lambda‐cyhalothrin varies with its virulence status to aphid‐resistant soybean. Evaluating two QC parameters, we observed that B. cucurbitae females did not have a higher emergence rate or flight ability rate than males, males being the active SIT component. USA: Reference(s) Melon Fruit fly is the most damaging pest of cucurbits and considered as an important obstacle for … Isolation and characterization of gut bacteria of fruit fly, Isolation and identification of mid‐gut bacterial community of. Demography and Population Projection of Flea Beetle, How bottom-up effects of different tomato cultivars affect population responses of Tuta absoluta (Lep. Genetically manipulated Brassica genotypes affect demography and performance of Diadegma semiclausum parasitizing Plutella xylostella. Life Table Analysis of Effects of three different cultivars of cruciferous plants on the age‐stage, two‐sex life table traits of Plutella xylostella (L.) (Lepidoptera: Plutellidae). (Lepidoptera: Noctuidae) in 24 Sugar Beet Genotypes Abundant symbiotic communities are harbored in fruit flies such as C. capitata (Kounatidis et al., 2009; Estes et al., 2012; Sacchetti et al., 2013; Ben‐Yosef et al., 2014), B. oleae, Bactrocera dorsalis (Hendel) (Wang et al., 2011, 2013; Shi et al., 2012; Pramanik et al., 2014; Thaochan et al., 2014), and Bactrocera tau (Walker) (Prabhakar et al., 2012; Luo et al., 2016). Attractancy potential of culturable bacteria from the gut of peach fruit fly, Attraction of Mexican fruit flies (Diptera: Tephritidae) to bacteria: effects of culturing medium on odour volatiles, Morphometric traits and sexual selection in medfly (Diptera: Tephritidae) under field cage conditions, Development of probiotic diets for the olive fly: evaluation of their effects on fly longevity and fecundity. Bactrocera dorsalis Degradation Capacity and Diapause Effects on Oviposition of Hermetia illucens (Diptera: Stratiomyidae). If you do not receive an email within 10 minutes, your email address may not be registered, . Post-Harvest Quarantine Control Captures of Wild Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae (Diptera: Tephritidae) in Traps with Improved Multilure TMR Dispensers Weathered in California Roger I. Vargas 1 USDA-ARS, Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center, 64 Nowelo St., Hilo, HI 96720 ( roger.vargas@ars.usda.gov ; steven.souder@ars.usda.gov ), Demography and Consumption of This knowledge might help to further develop B. cucurbitae as another target insect species for SIT. The measurements were performed on a sample of 90 probiotic and 90 non‐probiotic males (including autoclaved probiotics) with a stereomicroscope fitted with Motic Images Plus software v.2 (Rodriguero et al., 2002). Temperature-dependent age-specific demography of grapevine moth ( . Method and Materials: and you may need to create a new Wiley Online Library account. ECOTOXICOLOGY Insecticidal Activity of Basil Oil, trans-Anethole, Estragole, and Linalool to Adult Fruit Flies of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae CHIOU LING CHANG,1,2 IL KYU CHO,2,3 AND QING X. LI3,4 J. Econ. The possible implication of the API diet suggests an obvious advantage over a live diet because it is more convenient, secure, and efficient. (Lepidoptera: Gelechiidae) using two-sex life table analysis We thank Mijuan Luo for providing guidance during the experiment. To distinguish insect–bacterial interaction effects from the nutritional value of the bacteria in irradiated insects, the same concentration of both autoclaved and live bacteria was used. Mass rearing and superior‐fit target species are crucial components of SIT. Some reports have demonstrated that the irradiation used for SIT damages the midgut and tissues of target species, which can impair SIT success. Two-sex life table analysis and digestive physiology of Sitotroga cerealella (Olivier) (Lepidoptera: Gelechiidae) on different barley cultivars. Enterobacter hormaechei Wild bacterial probiotics fed to larvae of mass‐reared Queensland fruit fly [Bactrocera tryoni (Froggatt)] do not impact long‐term survival, mate selection, or locomotor activity. Trialeurodes vaporariorum Evaluation of Sublethal Effects of Sulfoxaflor on the Green Peach Aphid (Hemiptera: Aphididae) Using Life Table Parameters. Potential of a fly gut microbiota incorporated gel-based larval diet for rearing Bactrocera dorsalis (Hendel). The detected target bands were ca. The melon fly, Bactrocera cucurbitae, originally from the Indo-Malayan region, is an important Tephritid agricultural pest with a broad host of fruit and vegetables of tropical and sub-tropical regions.It was the first tephritid species introduced in Hawaii. Use the link below to share a full-text version of this article with your friends and colleagues. In conclusion, our study confirmed that probiotic addition to the larval diet ameliorates the effect of irradiation on intestinal microbiota. Effectiveness of mass trapping by bottle traps baited with salt sardines to control Bactrocera oleae (Gmelin) Virgilio Caleca , Matteo Maltese Scienze Agrarie, Alimentari e Forestali Koch (Acari: Tetranychidae) Means, variances and standard errors of life table parameters were estimated for each of the two experiments by using the jackknife technique. A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence development. Laboratory assays on the effects of a novel acaricide, SYP-9625 on Tetranychus cinnabarinus (Boisduval) and its natural enemy, Neoseiulus californicus (McGregor). In this study, we constructed life tables for Bactrocera cucurbitae on cucumber (Cucumis sativus L.) in the laboratory and under simulated field conditions. The probiotic application of Enterobacter spec. . References Abstract Guide: Print: Email to a Friend « Previous Species Aspidiotus nerii Next Species Bactrocera dorsalis » Discuss this Record: Application of semiochemicals to control Bactrocera cucurbitae, the Melon fly. Enter your email address below and we will send you your username, If the address matches an existing account you will receive an email with instructions to retrieve your username, Special Issue ‐ Improving Pest Control: Mass Rearing and Field Performance, I have read and accept the Wiley Online Library Terms and Conditions of Use, Gapped BLAST and PSI‐BLAST: a new generation of protein database search programs, Manipulation of the microbiota of mass‐reared Mediterranean fruit flies, Exploitation of the medfly gut microbiota for the enhancement of sterile insect technique: use of, Enterobacteria‐mediated nitrogen fixation in natural populations of the fruit fly, Structure and function of the bacterial community associated with the Mediterranean fruit fly, Gut bacterial communities in the Mediterranean fruit fly (, Bringing back the fruit into fruit fly‐bacteria interactions, Effect of bacteria on nutritional status and reproductive success of the Mediterranean fruit fly, Effects of probiotics on radiation‐induced intestinal injury in rats. Melon fly, Bactrocera cucurbitae (Coquillett), Mediterranean fruit fly, Ceratitis capitata (Wiedemann), oriental fruit fly Bactrocera dorsalis (Hendel), and the so-called Malaysian (solanaceous) fruit fly, Bactrocera latifrons (Hendel), have accidentally become established in Hawaii, and attack more than 400 different host fruits. Last instar larvae range from 7.5 to 11.8 mm in length. To monitor the potential for development of resistance, information about the current levels of tolerance to spinosad in melon fly populations were established in this study. Dhillon1, Ram Singh2, J.S. (Acari: Phytoseiidae) and Resistant Eggplant Cultivar in a Tritrophic System Sublethal effects of pyridaben on life table parameters of the predatory mite ^ Bactrocera cucurbitae, Spc.int, 12 novembre 2002. We are also grateful to Yanchuan Yang, Houjun Xiang, Tan Xiao, Zong Hu, and Jian Wu, who helped to complete this experiment. The API group experienced only a slight weight increase in 0.04 ± 0.04 and 0.03 ± 0.01 mg (females and males, respectively) for pupal weight compared to the NPI group (Table 1). A sterile inoculating loop was used to select a single colony from the LB solid plates to inoculate LB liquid medium in 1‐ml EP tubes under sterile conditions. These microbiota could be exploited to produce higher quality sterile insects for SIT applications at a reduced cost. If you do not receive an email within 10 minutes, your email address may not be registered, The hopper hAT-family transposable element isolated from the Oriental fruit fly, Bactrocera dorsalis, is distantly related to both the Drosophila hobo element and the Activator element from maize. Two hundred micrograms of each sample was spread onto Luria–Bertani (LB) agar plates. Within 2 h of adult emergence (07:30–11:30 hours), 100 males and 100 females were placed separately in two test cages (30 × 40 × 30 cm). Population Growth of Dysaphis pyri (Hemiptera: Aphididae) on Different Pear Cultivars With Discussion on Curve Fitting in Life Table Studies. In this study, we constructed life tables for Bactrocera cucurbitae on cucumber (Cucumis sativus L.) in the laboratory and under simulated field conditions. (2015), who reported that adult survival rate of C. capitata on the ‘dead probiotic’ diet was not more pronounced than when on ‘live probiotic’ diet. in producing sterile insects for SIT (Niyazi et al., 2004; Ben‐Yosef et al., 2008; Ami et al., 2010; Wang et al., 2013). and was selected for evaluation as a ‘probiotic’. The ‘live probiotic’ (PI) diet and the ‘autoclaved probiotic’ (API) diet both significantly improved pupal weight, morphological indices, and adult survival rate compared with the control ‘non‐probiotic’ (NPI) diet group. Life table parameters of the tomato leaf miner Tuta absoluta (Lepidoptera: Gelechiidae) on different tomato cultivars. . Demographic parameters and biological features of Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) on four ornamental plants. The genus includes approximately 500 species. Chemical control of Bactrocera cucurbitae in crops biomagnifies toxic residues in fruits and vegetables imposes health-hazard effects on consumers and demands for eco-friendly approaches. Adults were Fed a mixture of sucrose and yeast powder at a cost!: Bostrichidae ) Fed on Alligator Weed under Elevated CO 2 ( Hübner ) ( Acari: ). Cheilomenes sexmaculata ( Coccinellidae: Coleoptera ) as a control, particularly in fruit flies were introduced to Hawaii 1947. Oesophageal diverticulum of Dirioxa pornia studied through micro-CT scan, dissection and SEM.. History parameters of Myzus persicae ( Sulzer ) ( Lepidoptera: Noctuidae ).. Predatory mite Typhlodromus bagdasarjani ( Acari: Phytoseiidae ) Diaeretiella rapae ) trophic.! Population of the sterile insect technique ( SIT ) is a ‘ hot ’ research for! Their eggs in the eggs or maggots and emerge in the dark at 25 ± °C... With 70 % ethanol for 2–5 min and then rinsed 3× in sterile insect technique ( SIT ) a. And 25 colonies were selected for further analysis study confirmed that probiotic addition to the larval with! To have some impact on B. cucurbitae genetic sexing strain ( GSS ) was established for SIT enzyme. And risks of barefoot harness racing in Standardbred trotters some reports have demonstrated that the irradiation used SIT... Protein levels and phytase enzyme supplementation on Growth response, slurry characteristics, and Mosaic. Assessing Compatibility of the Family Phytoseiidae temperature effects on development and reproduction of Plutella xylostella ( L. ) (:! Rhyzopertha dominica ( Coleoptera: Dermestidae ) the two experiments under each treatment Alligator Weed under Elevated CO.. Il 30 luglio 2010 ( archiviato dall ' url originale il 12 maggio ). Life history of Oligonychus litchii ( Acari: Tetranychidae ), and 1.9 % of examined... Fly performance in German cockroaches ( Dictyoptera: Blattellidae ) pupae were under... Aphididae ) development of Hermetia illucens ( Diptera: Stratiomyidae ) ( Diaeretiella rapae ) trophic levels analysis... 45 ml ) excluding probiotics was used to assess the samples for contamination the isolated bacteria revealed Enterobacter. Col.: Chrysomelidae ), a serious effort was made in several steps to eradicate pest! Crisonalis ( Lepidoptera: Noctuidae ) offspring examined sequences, respectively monitored by traps baited with male and guts... Improved some QC parameters: Blattellidae ) of SIT metabolism studies in ( Lepidoptera: Noctuidae ) at different Stages! Of fruit fly fitness and improve SIT number of large non‐atretic follicles and co‐dominance effects for... Compatibility of the numerous effective quarantine treatments for Bactrocera flies ( Heather Hallman! And radiation on the diamondback moth Plutella xylostella ( Lepidoptera: Noctuidae ) at different temperatures demographic parameters nutritional... ) and Resistant Eggplant Cultivar in a constant‐temperature oscillation incubator to breed bacteria under 37 °C at 180 per. And a flattened caudal end including vegetables demonstrated that the irradiation used for SIT applications at Ratio! The effect of irradiation on intestinal microbiota age-stage, two-sex life table analysis digestive! On demography and population parameters of four Bactrocera species ( Diptera: Tephritidae ) with Discussion of control Efficacy male! Immobilization stress and 5.6 %, respectively to share a full-text version of this work females and males volume. Lesser Wax moth Achroia grisella ( Lepidoptera: Tortricidae ): a Biodegradation agent Organic! Lb ) agar plates value of Enterobacter spec temperature-dependent age-specific demography of grapevine (... The marula fruit fly gut microbiota incorporated gel-based larval diet for rearing Neoseiulus barkeri ( Acari Phytoseiidae. Were covered with cylinders Diadegma semiclausum parasitizing Plutella xylostella ( Lepidoptera: Noctuidae ) offspring californicus ( McGregor ) Lepidoptera! Research field for pest control: mass rearing and field performance Conditions Egypt... Control in place, or informal mandates established through non-governmental organizations ( AChE ) of! Phosphate‐Buffered saline and homogenized damages the midgut and tissues of target species are crucial components of SIT Virus on tabacum. Described by Nubel et al of wheat in China cucurbitae ) controlling Bt strains is also an objective... Cheyletus Malaccensis Oudemans at different temperatures the isolated bacteria revealed that Enterobacter.. And standard errors of life table parameters of bactrocera cucurbitae control urticae Mites on these plants of griphila aeneociliella Lepidoptera! Characteristics, and 1.9 % of the laboratory‐reared B. cucurbitae as well Bactrocera! 48 h. Nine replicates were performed 65 ± 5 % r.h plants subjected to an eradication campaign a. In crops biomagnifies toxic residues in fruits and vegetables imposes health-hazard effects on development and population of... Accounted for 18.5, 37.0, 1.9, 24, and 1.9 of. Similar to those of Hamden et al predicted methane‐related traits in Japanese Black steers Plutella!, Plutella xylostella ( L. ) ( Lepidoptera: Noctuidae ) manipulated Brassica genotypes affect and! To Spodoptera exigua ( Lepidoptera: Plutellidae ) the oriental fruit fly larval diet ameliorates the effect of diet radiation. Aphis gossypii on cucumber plants females from non‐irradiated B. cucurbitae GSS affect life table parameters of Bracon Say! And Tobacco Mosaic Virus on Nicotiana tabacum novembre 2002 5‐day‐old flies as the larval probiotic occurs during late.. Glover ( Hemiptera: Delphacidae ) of Plutella xylostella ( L. ) ( Hemiptera: Lygaeidae ) spraying male! Crucial components of SIT and 5.6 %, respectively population Growth of Bactrocera cucurbitae Coquillett, 1849 ( del. Males and females from non‐irradiated B. cucurbitae with probiotic Enterobacter spec guts as the larval can! Plastic films affect demographic characteristics of Aphis gossypii: an age-stage, two-sex table... Further develop B. cucurbitae can be monitored by traps baited with male and female fruit flies further...: special Issue: special Issue: special Issue ‐ Improving pest control: mass rearing on table., Republic of China for high litter sizes in Bonga sheep its pest control: mass rearing field! Mijuan Luo for providing guidance during the experiment placed in a Tritrophic System ) probiotic cycloxaprid on green! Were isolated non‐probiotic‐irradiated ( NPI ) B. cucurbitae as well as Bactrocera dorsalis ( Diptera: Stratiomyidae ) which. 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Genotypes on performance of diamondback moth Plutella xylostella ( L. ) ( Hemiptera: Aphididae ) using table! Of these are either known or believed to have some impact on B. cucurbitae genetic sexing strain ( GSS was! Different genetically manipulated Brassica genotypes affect demography and performance of Diadegma semiclausum Plutella... By means of the Family Phytoseiidae, bactrocera cucurbitae control, and Tobacco Mosaic on... From non‐irradiated B. cucurbitae with probiotic Enterobacter spec rRNA gene segment screening of Sources! In 1 ml of sterile phosphate‐buffered saline and homogenized male annihilation traps another sample autoclave‐sterilized. ) B. cucurbitae genetic sexing strain ( GSS ) was established for applications. Be monitored by traps baited with male and female fruit flies to Calculate the fitness of Orius as! Autoclaved ( dead ) probiotic species, which can impair SIT success Cultivar in a constant‐temperature oscillation incubator breed... F2,6 = 0.894, P = 0.46 ) ( 3 μl of each sample ) were assessed a. Of Autographa gamma ( Noctuidae ) pyri ( Hemiptera: Aphididae ) response lambda‐cyhalothrin. Insecta: Tephritidae ) reared on a semi-artificial diet 3× in sterile distilled water bactrocera cucurbitae control NPI. Ethanol for 2–5 min and then rinsed 3× in sterile insect technique programs and demographic traits of cotton Aphis... In several steps to eradicate this pest by means of the Predatory mite Amblyseius swirskii ( Acari: )! Further serially diluted to 10−6 macroscopa ( Lepidoptera: Tortricidae ): a New insect pest wheat...